Yeo was born in Singapore in 17 April 1977, she studied in Fairfield Methodist Primary School and Nanyang Girls’ High School before moving to Dayton, Ohio, for four years, where her father was posted as an engineer in the Republic of Singapore Air Force. She completed her secondary education at Dayton Christian High School. On returning to Singapore, she entered Ngee Ann Polytechnic and studied mass communications, because her certificate from America could not be recognised in Singapore therefore she could not take her A levels at a junior college but eventually graduating in 1998 from Ngee Ann Polytechnic.

Yeo began dating DJ Glenn Ong in 2001 and the couple married in New Creation Church three years later in 2004, but separated in early 2009. In 2010 Yeo remarried to Thorsten Nolte, a British immigrant in Singapore; the couple have a daughter. Jamie Yeo and Thorsten Nolte divorced in February 2015. In April 2016, Jamie gained sole custody of her daughter, Alysia. In June 2017, she announced to the press that she was getting remarried for the third time to another British immigrant Rupert, and was five months pregnant with a baby boy. Her son, Luke, was born on 29 August 2017.

Yeo began as an actress portraying Tammy in the TV show Growing Up, arguably the role that made her famous. She has also acted in MediaCorp Ch8 Mandarin language productions Holland V and Baby Boom in 2003.

In 2005, Jamie started with ESPN and became the face of shows like Nokia Football Crazy and Football Extra. In 2009 Nokia Football Crazy was renamed Castrol Football Crazy due to changes in sponsorship and it went off air in 2010. In an interview with TheSportsCampus.com in 2009, Jamie shared that her favourite football club is Arsenal F.C., and her favourite player is Thierry Henry.

In 2012, Yeo went back to radio with SAFRA Radio on the Singapore station Power98FM.

In 2015, Jamie returned to Mediacorp as Radio DJ for Gold 905FM. In March 2016, she took over the drive-time show, “The Homestretch”.