Jo Ann Pflug was born in Atlanta, to J. Lynn and Kelly Pflug. She was raised in Winter Park, Florida, where her father was elected mayor in 1958, and graduated from Winter Park High School, Rollins College, and the University of Miami, receiving her BA in broadcasting and her minor in American history.

She also appeared in Catlow (1971) with Yul Brynner, and Where Does It Hurt? (1972) starring Peter Sellers. Her other notable roles include the voice of Invisible Girl in the 1967 animated version of Fantastic Four, Lt. Katherine O’Hara in the television series spin-off of Operation Petticoat, Boss Jack’s wife in Traveller (1997), and Cynthia Vaughn Midnight in the Garden of Good and Evil (1997).

Pflug’s first major role was as U.S. Army nurse Lt. Maria “Dish” Schneider in the 1970 film M*A*S*H. Also in 1970, she starred in an episode of Marcus Welby, M.D. titled “To Carry the Sun in a Golden Cup”, in which she played an ailing nurse.

Pflug married Chuck Woolery in 1972 at the Knowles Memorial Chapel at Rollins College in Orlando, Florida. They had a daughter, Melissa. The couple divorced in 1980.

In 1984, she was the first actress to play Taylor Chapin in the syndicated soap opera Rituals. She made guest appearances on many television series including McCloud, The Love Boat, CHiPs, The Dukes of Hazzard, One Day at a Time, Knight Rider, Love, American Style, Adam-12, Quincy, M.E., Alias Smith and Jones, Charlie’s Angels and The Colbys.

A resident of Tequesta, Florida, in 2011 she interviewed Pat Boone, Shirley MacLaine and other celebrities for The Jo Ann Pflug Show on Seaview Radio.