He attended Curtin University in Perth, Australia, majoring in journalism and obtained a Bachelor of Arts in English. He graduated in 1988

On 12 June 2010, Iskander married doctor and FHM model Jezamine Lim after meeting her through Facebook.

In 2016, Iskander was one of 89 international stand-up comedians nominated for Laugh Factory’s Funniest Person in the World. Iskander has been announced as an ambassador for Culture and Tourism Malaysia by Datuk Seri Nazri Aziz (Tourism and Culture Minister) as he is now one of the attraction in Malaysia.

After being crowned as the Laugh Factory’s Funniest Person in the World in 2016, in 2017, Iskander curated the Kuala Lumpur International Comedy Festival (KLICFest 2017) which considered by many to be Asia’s best comedy showcase. The festival programme featured performances from the finest Malaysian acts, the biggest international stars and one local legend offering both English and Bahasa Malaysia comedy.

Thereafter the kick-off show (KLICFest) in Kuala Lumpur, in 2018, Harith Iskander, in partnership with Celcom, had tour his home ground again to deliver the laughs – the #KitaOK Live Comedy Tour 2018. The tour started off in Kuala Lumpur before showing at six other cities in Malaysia: including Johor, Malacca, Penang, Kuching, Kota Kinabalu and Labuan. Harith Iskander also succeeded in putting Malaysia on the comedy map the second time by releasing his Netflix original comedy special, I Told You So in 2018.

Apart from that in 2018, Harith held his Australia tour named Harith Iskander The Tour – Melbourne International Comedy Festival 2018, Adelaide Fringe 2018 and Perth Fringe 2018. Besides, Harith tickled the audiences in the City Of Angels (Los Angeles) in June 2017 where the organizer was The Laugh Factory.