In 1983, Lee premiered his film independent short film titled, Joe’s Bed-Stuy Barbershop: We Cut Heads. Lee submitted the film as his master’s degree thesis at the Tisch School of the Arts. Lee’s classmates Ang Lee and Ernest R. Dickerson worked on the film as assistant director and cinematographer, respectively. The film was the first student film to be showcased in Lincoln Center’s New Directors New Films Festival. Lee’s father, Bill Lee, composed the score. The film won a Student Academy Award.

In 1983, Lee won the Student Academy Award for his film Joe’s Bed-Stuy Barbershop: We Cut Heads. He won awards at the Black Reel Awards for Love and Basketball, the Black Movie Awards for Inside Man, and the Berlin International Film Festival for Get on the Bus. He won BAFTA Award for Best Adapted Screenplay for BlacKkKlansman.

In 1985, Lee began work on his first feature film, She’s Gotta Have It. The film filmed in black-and-white, concerns a young woman (Johns) who is seeing three men, and the feelings this arrangement provokes. The film was Lee’s first feature-length film that launched Lee’s career. Lee wrote, directed, produced, starred and edited the film with a budget of $175,000, he shot the film in two weeks. When the film was released in 1986, it grossed over $7 million at the U.S. box office. New York Times film critic A.O. Scott wrote that the film “ushered in (along with Jim Jarmusch’s Stranger Than Paradise) the American independent film movement of the 1980s. It was also a groundbreaking film for African-American filmmakers and a welcome change in the representation of blacks in American cinema, depicting men and women of color not as pimps and whores, but as intelligent, upscale urbanites.”

In 1989, Lee made perhaps his most seminal film, Do the Right Thing, which focused on a Brooklyn neighborhood’s simmering racial tension on a hot summer day. The film’s cast included Lee, Danny Aiello, Bill Nunn, Ossie Davis, Ruby Dee, Giancarlo Esposito, Rosie Perez, John Turturro, Martin Lawrence and Samuel L. Jackson. The film gained critical acclaim as one of the best films of the year from film critics including both Gene Siskel and Roger Ebert who ranked the film as the best of 1989, and later in their top 10 films of the decade (No. 6 for Siskel and No. 4 for Ebert). Ebert later added the film to his list of The Great Movies.

After the 1990 release of Mo’ Better Blues, Lee was accused of antisemitism by the Anti-Defamation League and several film critics. They criticized the characters of the club owners Josh and Moe Flatbush, described as “Shylocks”. Lee denied the charge, explaining that he wrote those characters in order to depict how black artists struggled against exploitation. Lee said that Lew Wasserman, Sidney Sheinberg, or Tom Pollock, the Jewish heads of MCA and Universal Studios, were unlikely to allow antisemitic content in a film they produced. He said he could not make an antisemitic film because Jews run Hollywood, and “that’s a fact”.

In 1991, Lee taught a course at Harvard about filmmaking. In 1993, he began to teach at New York University’s Tisch School of the Arts in the Graduate Film Program. It was there that he received his master of fine arts. In 2002 he was appointed as artistic director of the school. He is now a tenured professor at NYU.

In 1992, Spike released his biographical epic film Malcolm X based on the Autobiography of Malcolm X, starring Denzel Washington as the famed civil rights leader. The film dramatizes key events in Malcolm X’s life: his criminal career, his incarceration, his conversion to Islam, his ministry as a member of the Nation of Islam and his later falling out with the organization, his marriage to Betty X, his pilgrimage to Mecca and reevaluation of his views concerning whites, and his assassination on February 21, 1965. Defining childhood incidents, including his father’s death, his mother’s mental illness, and his experiences with racism are dramatized in flashbacks. The film received widespread critical acclaim including from critic Roger Ebert ranked the film No. 1 on his Top 10 list for 1992 and described the film as “one of the great screen biographies, celebrating the sweep of an American life that bottomed out in prison before its hero reinvented himself.” Ebert and Martin Scorsese, who was sitting in for late At the Movies co-host Gene Siskel, both ranked Malcolm X among the ten best films of the 1990s. Denzel Washington’s portrayal of Malcolm X in particular was widely praised and he was nominated for the Academy Award for Best Actor. Washington lost to Al Pacino (Scent of a Woman), a decision which Lee criticized, saying “I’m not the only one who thinks Denzel was robbed on that one.”

Lee met his wife, attorney Tonya Lewis, in 1992, and they were married a year later in New York. They have one daughter, Satchel, born in 1994, and a son, Jackson, born in 1997.

His 1997 documentary 4 Little Girls, about the girls killed in the 16th Street Baptist Church bombing in Birmingham, Alabama, in 1963, was nominated for the Academy Award for Best Feature Documentary. In 2017, the film was selected for preservation in the United States National Film Registry by the Library of Congress as being “culturally, historically, or aesthetically significant”.

In May 1999, the New York Post reported that Lee made an inflammatory comment about Charlton Heston, president of the National Rifle Association, while speaking to reporters at the Cannes Film Festival. Lee was quoted as saying the National Rifle Association should be disbanded and, of Heston, someone should “Shoot him with a .44 Bull Dog.” Lee said he intended it as a joke. He was responding to coverage about whether Hollywood was responsible for school shootings. “The problem is guns”, he said. Republican House Majority Leader Dick Armey condemned Lee as having “nothing to offer the debate on school violence except more violence and more hate”.

In 2002, Lee directed 25th Hour starring Edward Norton, and Philip Seymour Hoffman which opened to positive reviews, with several critics since having named it one of the best films of its decade. Film critic Roger Ebert added the film to his “Great Movies” list on December 16, 2009. A. O. Scott, Richard Roeper and Roger Ebert all put it on their “best films of the decade” lists. It was later named the 26th greatest film since 2000 in a BBC poll of 177 critics. The film was also a financial success earning almost $24 million against a $5 million budget.

In June 2003, Lee sought an injunction against Spike TV to prevent them from using his nickname. Lee claimed that because of his fame, viewers would think he was associated with the new channel.

In October 2005, Lee responded to a CNN anchor’s question as to whether the government intentionally ignored the plight of black Americans during the 2005 Hurricane Katrina catastrophe by saying, “It’s not too far-fetched. I don’t put anything past the United States government. I don’t find it too far-fetched that they tried to displace all the black people out of New Orleans.” In later comments, Lee cited the government’s past including the Tuskegee Study of Untreated Syphilis in the Negro Male.

In 2006, Lee directed Inside Man starring Denzel Washington, Jodie Foster, and Clive Owen, Chiwetel Ejiofor, Willem Dafoe and Christopher Plummer. The film was an unusual film for Lee considering it was a studio heist thriller. The film was a critical and financial success earning $186 million off a $45 million budget. Empire gave the film four stars out of five, concluding, “It’s certainly a Spike Lee film, but no Spike Lee Joint. Still, he’s delivered a pacy, vigorous and frequently masterful take on a well-worn genre. Thanks to some slick lens work and a cast on cracking form, Lee proves (perhaps above all to himself?) that playing it straight is not always a bad thing.”

On May 2, 2007, the 50th San Francisco International Film Festival honored Spike Lee with the San Francisco Film Society’s Directing Award. In 2008, he received the Wexner Prize. In 2013, he won The Dorothy and Lillian Gish Prize, one of the richest prizes in the American arts worth $300,000.

At the 2008 Cannes Film Festival, Lee, who was then making Miracle at St. Anna, about an all-black U.S. division fighting in Italy during World War II, criticized director Clint Eastwood for not depicting black Marines in his own World War II film, Flags of Our Fathers. Citing historical accuracy, Eastwood responded that his film was specifically about the Marines who raised the flag on Mount Suribachi at Iwo Jima, pointing out that while black Marines did fight at Iwo Jima, the U.S. military was racially segregated during World War II, and none of the men who raised the flag were black. He angrily said that Lee should “shut his face”. Lee responded that Eastwood was acting like an “angry old man”, and argued that despite making two Iwo Jima films back to back, Letters from Iwo Jima and Flags of Our Fathers, “there was not one black soldier in both of those films”. He added that he and Eastwood were “not on a plantation”. Lee later claimed that the event was exaggerated by the media and that he and Eastwood had reconciled through mutual friend Steven Spielberg, culminating in his sending Eastwood a print of Miracle at St. Anna.

In March 2012, after the shooting of Trayvon Martin, Spike Lee was one of many people who used Twitter to circulate a message that claimed to give the home address of the shooter George Zimmerman. The address turned out to be incorrect, causing the real occupants, Elaine and David McClain, to leave home and stay at a hotel due to numerous death threats. Lee issued an apology and reached an agreement with the McClains, which reportedly included “compensation”, with their attorney stating “The McClains’ claim is fully resolved”. Nevertheless, in November 2013, the McClains filed a negligence lawsuit which accused Lee of “encouraging a dangerous mob mentality among his Twitter followers, as well as the public-at-large”. The lawsuit, which a court filing reportedly valued at $1.2 million, alleged that the couple suffered “injuries and damages” that continued after the initial settlement up through Zimmerman’s trial in 2013. A Seminole County judge dismissed the McClains’ suit, agreeing with Lee that the issue had already been settled previously.

In 2015, Lee received an Academy Honorary Award from the Academy of Motion Picture Arts and Sciences for his contributions to film. Friends and frequent collaborators Wesley Snipes, Denzel Washington, Samuel L. Jackson presented Lee with the award at the private Governors Awards ceremony.

Lee was nominated for Academy Awards for Best Original Screenplay for Do the Right Thing and Best Documentary for 4 Little Girls, but did not win either award. In November 2015, he was given the Academy Honorary Award for his contributions to filmmaking. In 2019, he received his first Best Picture and Best Director nominations.

In 2015, at the age of 58, Lee became the youngest person ever to receive an Honorary Academy Award. Lee received the award as “a champion of independent film and an inspiration to young filmmakers”. Frequent collaborators Denzel Washington, Samuel L. Jackson, and Wesley Snipes presented Lee with the award at a private ceremony at the Governors Awards.

On May 18, 2016, Lee delivered the Commencement address for The Johns Hopkins University Class of 2016.

Lee’s 2018 film BlacKkKlansman, a true crime drama set in the 1970s centered around the true story of a black police officer, Ron Stallworth infiltrating the Ku Klux Klan. The film premiered at the 2018 Cannes Film Festival, where it won the Grand Prix and opened the following August. The film received near universal praise when it opened in North America receiving a 96% on Rotten Tomatoes with the critics consensus reading, “BlacKkKlansman uses history to offer bitingly trenchant commentary on current events – and brings out some of Spike Lee’s hardest-hitting work in decades along the way.” In 2019, during the awards season leading up to the Academy Awards, Lee was invited to join a Directors Roundtable conversation run by The Hollywood Reporter. The roundtable included Ryan Coogler (Black Panther), Yorgos Lanthimos (The Favourite), Alfonso Cuarón (Roma), Marielle Heller (Can You Ever Forgive Me?), and Bradley Cooper (A Star is Born). It was nominated for the Academy Award for Best Picture and Best Director (Lee’s first ever nomination in this category). Lee won his first competitive Academy Award in the category Best Adapted Screenplay. When asked by journalists from the BBC if the Best Picture winner Green Book offended him, Lee replied, “Let me give you a British answer, it’s not my cup of tea”. Many journalists in the industry noted how the 2019 Oscars with BlacKkKlansman competing against eventual winner Green Book mirrored the 1989 Oscars with Lee’s film Do the Right Thing missing out on a Best Picture nomination over the eventual winner Driving Miss Daisy.

In 2018, during an interview with GQ, Lee cited some of his favorite films as Elia Kazan’s On the Waterfront (1954) and A Face in the Crowd (1957), as well as Martin Scorsese’s Mean Streets (1973). Lee says that he befriended Scorsese after attending a screening of After Hours at NYU.

Two of his films have competed for the Palme d’Or award at the Cannes Film Festival, and of the two, BlacKkKlansman won the Grand Prix in 2018.

In 2019, Lee’s film BlacKkKlansman went on to receive 6 Academy Award nominations. Lee himself was nominated for 3 Oscars for Lee for Best Picture, Best Director, and Best Adapted Screenplay. He went on to win the Best Adapted Screenplay, his first Academy Award.

Lee’s Vietnam war film Da 5 Bloods was released on Netflix. The film starred Delroy Lindo, Jonathan Majors, Clarke Peters, Isiah Whitlock Jr., Mélanie Thierry, Paul Walter Hauser and Chadwick Boseman. The film was released worldwide on June 12, 2020. The film’s plot follows a group of aging Vietnam War veterans who return to the country in search of the remains of their fallen squad leader, as well as the treasure they buried while serving there. Prior to the COVID-19 pandemic, the film was originally scheduled to premiere out-of-competition at the 2020 Cannes Film Festival, then play in theaters in May or June before streaming on Netflix. The film received widespread critical acclaim with the website Rotten Tomatoes’ approval rating being 92% based on 252 reviews, with the critical consensus reading: “Fierce energy and ambition course through Da 5 Bloods, coming together to fuel one of Spike Lee’s most urgent and impactful films.” On Metacritic, the film has a weighted average score of 82 out of 100, based on 49 critics, indicating “universal acclaim”.

In May 2020, he published a 3-minute short film titled NEW YORK NEW YORK on Instagram that was later featured on the city’s official website.

In March 2020, a video of Spike was released on Twitter showing the director having an altercation with the security team near the elevators at Madison Square Garden. Speculation arose as to whether Spike was being removed from the building. The New York Knicks released a statement saying, “The idea that Spike Lee is a victim because we have repeatedly asked him to not use our employee entrance and instead use a dedicated VIP entrance — which is used by every other celebrity who enters The Garden — is laughable. He is welcome to come to The Garden anytime via the VIP or general entrance; just not through our employee entrance, which is what he and Jim (James Dolan) agreed to [Monday] night when they shook hands.” Spike refuted Dolan’s story alleging that he had been using the same entrance for the past 28 years. Lee stated he wouldn’t attend the rest of the games for the season.

In June 2020, Lee defended filmmaker Woody Allen despite his sexual abuse allegation during a radio interview stating: “I’d just like to say Woody Allen is a great, great filmmaker and this cancel thing is not just Woody. And I think when we look back on it we are going to see that short of killing somebody, I don’t know you that you can just erase somebody like they never existed. Woody’s a friend of mine. I know he’s going through it right now.” Following social media backlash, Lee issued an apology on Twitter.