Toñi Moreno was born in El Prat de Llobregat, when her father worked at SEAT, but at age 8 her family moved to Cádiz. She later studied law there, although her vocation was journalism. In 1985, at age 12, Moreno began her radio career on the station Radio Sanlúcar. At age 14, she left the world of voiceover to begin working at Telesanlúcar, the local television station in Sanlúcar de Barrameda. There she made her on-air debut. Later she joined Canal Sur, where she was a reporter for the program Andalucía directo [es]. Since then, she has had an extensive professional career in television, working on shows linked to the regional autonomy of Andalusia such as Tiene arreglo [es] and 75 minutos, which she was on for eight years. After her departure for private television, on Antena 3 she hosted the reality show Libertad vigilada [es], coordinated the morning magazine Cada día, and worked as a reporter. On Cuatro, she headed the show 1 Equipo.

After years of success on the Andalusian station Canal Sur, Televisión Española (TVE) signed her to host Entre todos [es], a program of solidarity and citizen participation – the national version of Tiene arreglo. She presented this from 26 August 2013 to 27 June 2014 on La 1. With the start of the new television season on 15 September, she returned to the same channel with another afternoon program called T con T [es] that succeeded the canceled Entre todos. However, two months later, it would be removed from the programming schedule due to low ratings. In June 2015, Moreno began working on the Canal Sur program Los descendientes, which enjoyed critical and public success. Since January 2017, she has also hosted Gente maravillosa [es], a magazine that tries to empower people who show positive values in everyday situations.

Toñi Moreno was romantically linked to journalist María Casado Paredes [es] by the newspaper Vozpópuli in 2016, though she has repeatedly refused to discuss her private life with the media.

In April 2017 she returned to Antena 3 to present a set of interviews entitled El árbol de tu vida [es]. While this program was still on the air, Telecinco announced signing her to host Viva la vida [es], a weekend evening magazine. She simultaneously presents this with Gente maravillosa on Canal Sur.

On 20 October 2018, the Mediaset leadership decided to remove Emma García [es] from Mujeres y Hombres y Viceversa, to be replaced by Moreno on 5 November. In this way, García would come to present Viva la vida.